Lysozyme

Summary

Lysozyme is a glycoside hydrolase that specifically acts on the cell wall of microorganisms. It is ubiquitously present in various tissues and secretions of humans and animals, as well as in certain plants and microorganisms. It can dissolve the cell walls of Gram-positive bacteria. It has a strong killing effect and can also be used in crystalline protein lysozyme preparations to prevent food deterioration. It has a wide range of applications in food preservation, medicine, daily chemicals, baby food and scientific research.

 

Theory

Lysozyme mainly breaks down the β-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the cell wall, so that insoluble mucopolysaccharides in the cell wall are decomposed into soluble glycopeptides, causing the ruptured cell wall to escape the contents And dissolve the bacteria. The lysozyme eliminates and directly binds to the minus-minus virus protein, and forms a double salt with DNA, RNA, and apoprotein to inactivate the virus. Therefore, the enzyme has antibacterial, anti-inflammatory, and antiviral effects.

 

Characteristics
1. Temperature range: the effective temperature range of lysozyme is 30-50℃, and the optimum temperature range is 30-37℃;
2. PH value range: the effective pH range is 3.5-8.5, and the most suitable PH value range is 5.3-6.4;
3. The influence of metal ions on enzyme activity: It is susceptible to the influence of Fe3+, Hg+, Pb+ and other metal ions, which reduces the activity. Mg2+ and Ca2+ have a certain degree of inhibitory effect on enzyme activity.

Character
1. Product specifications: Lysozyme is light yellow or white solid powder, enzyme activity 1000u/mg; solid enzyme fineness (0.4mm standard sieve pass rate): ≧80%.
2. Definition of enzyme activity: Under the measurement conditions (25°C; pH value 6.2), at a wavelength of 450nm, the absorbance decreases by 0.001 per minute as an enzyme activity unit.
3. Product standard: the implementation of the people's Republic of China 30990-2014  industry standard GB/T.

Application
1. Lysozyme is a non-toxic and side-effect protein, and has a certain bacteriolytic effect, so it can be used as a natural food preservative. It has been widely used in the preservation of aquatic products, meat food, cakes, sake, cooking wine and beverages; it can also be added to milk powder to emulsify cows and milk to inhibit the survival of spoilage microorganisms in the intestinal tract, while directly or indirectly promoting Proliferation of bifidobacteria in the intestine.
2. As a non-specific immune factor that exists in normal body fluids and tissues of the human body, lysozyme has a variety of pharmacological effects. It has antibacterial, antiviral and antitumor effects. The current medical lysozyme is indicated for bleeding and hematuria , Blood sputum and rhinitis.
3. Lysozyme has the function of destroying the structure of the bacterial cell wall. G+ bacteria are treated with this enzyme to obtain protoplasts. Therefore, lysozyme is an essential tool enzyme for cell fusion operations in genetic engineering and cell engineering.

Packaging And Storage
The solids are packed in food packaging bags, 20kg/bag (packaged according to customer requirements).

Lysozyme is recommended to store in a cool and dry place, with a relative humidity of less than 60% and a temperature of less than 25°C; the shelf life is 12 months. If placed in a cool, low temperature, dry environment at 2~8℃, the shelf life is 24 months. It is best to store away from odorous substances.

 

CERTIFICATE OF ANALYSIS

 

Product Name Lysozyme    
Other Name N/A Country of Origin China
Source N/A Manufacture Date /
Batch Number / Expiration Date /
Package 25 kg/bucket Quantity /
PROTOCOL SPECIFICATIONS RESULTS METHOD
PHYSICAL & CHEMICAL ANALYSIS
Description White powder Complies Visual
Odor & Taste Characteristic odor and taste Complies Taste
Moisture NMT 7% 3.8% Moisture Analyzer
IDENTIFICATION
Identifiable Activity Positive for Bromelain Activity Complies In-house
ACTIVITY
Lysozyme Activity NLT 20,000 U/mg 22,100 U/mg In-house
MICROBIOLOGICAL
Total bacteria count
Coliform bacteria (CFU/g)
Molds and Yeasts
E.Coli
Salmonella
Staphylococcus aureus
Pseudomonas aeruginosa
NMT 1,000 CFU/g
NMT 30 CFU/g
NMT 100 CFU/g
Absent
Absent
Absent
Absent
10 CFU/g
<10 CFU/g
<10 CFU/g
Not Detected
Not Detected
Not Detected
Not Detected
FDA BAM Online Ch.3
FDA BAM Online Ch.4
FDA BAM Online Ch. 2
FDA BAM Online Ch.4
FDA BAM Online Ch.5
FDA BAM Online Ch.12
AOAC
HEAVY METALS
Lead
Mercury
Cadmium
Arsenic
NMT 3 ppm
NMT 0.1 ppm
NMT 1 ppm
NMT 1 ppm
<3 ppm
<0.1 ppm
<1 ppm
<1ppm
USP <231>
USP <231>
USP <231>
USP <231>
Storage: Store in cool and dry place protected from light, keep drum close when not in use.
Conclusion: Conforms to IU Standards.
Shelf Life: Shelf life of lysozymeunder prescribed storage conditions and air-tight packing will be 2 years.
The product COMPLIES with the above said specifications
Approved by

 

*The above information is a direct translation of the information provided to Newgen Biotech from the manufacturer of this product. This Certificate of Analysis should be used for informational purposes and is not intended as a substitute for strict quality control analysis by the purchaser of lysozyme.


Packaging and storage

Solid food packaging bag, 25 kg / barrel.

 

Lysozyme should be stored in dry, cool and well ventilated places away from the sun, heat.

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